A system for high-speed microinjection of adherent cells

Wenhui Wang, Yu Sun, Ming Zhang, Robin Anderson, Lowell Langille, Warren Chan

Review of Scientific Instruments 79, 104302 (2008) |  DOI: 10.1063/1.3006000

Abstract

This paper reports on a semi-automated microrobotic system for adherent cell injection. Different from embryos/oocytes that have a spherical shape and regular morphology, adherent cells are flat with a thickness of a few micrometers and are highly irregular in morphology. Based on computer vision microscopy and motion control, the system coordinately controls a three-degrees-of-freedom microrobot and a precision